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hypR [2018-02-13 15:39:49]
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hypR [2018-02-13 15:39:49]

MarR/DUF24 family transcription regulator, positively controls the nitroreductase gene hypO in response to disulfide stress
locus
BSU40540
pI
8.41
mw
14.59 kDa
protein length
125 aa Sequence Blast
gene length
375 bp Sequence Blast
function
control of the nitroreductase gene hypO in response to disulfide stress (diamide, NaOCl)
product
MarR/DUF24 family transcription regulator HypR
essential
no
synonyms
yybR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    4,167,622 → 4,167,999

    The protein

    Protein family

  • [[MarR/DUF24 family]]

    • Paralogous protein(s)

  • [protein|51B1913B59C1876CCED4A88414797B3A22BE4E3C|YdeP], [protein|C57DDC9710CB557179B062F9C5D786DB02A3B5FC|YkvN], [protein|E757C4B329A99E3D0C70C77437DF4DDA300CA5E7|YdzF]

    • Kinetic information

  • Cys14 redox sensing Cys, has lower pKa of 6.36 [Pubmed|22238377]

    • [SW|Domains]

  • 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface [Pubmed|22238377]

    • Modification

  • oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress [Pubmed|22238377]
  • Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR [Pubmed|22238377]

    • Structure

  • [PDB|4A5N] (reduced HypRC14S dimer) [Pubmed|22238377]
  • [PDB|4A5M] (oxidized HypR C14-C49' intersubunit disulfide-linked dimer ) [Pubmed|22238377]

    • [SW|Localization]

  • cytoplasmic

    • Expression and Regulation

    Operons

    genes
    [gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]
    description
    [Pubmed|22238377]

    regulatory mechanism

  • [protein|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|HypR]: activation, (autoregulation)[Pubmed|22238377], in [regulon|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|HypR regulon]

    • regulation

  • activated by disulfide stress conditions (diamide, NaOCl) ''in vivo'' and ''in vitro '' (autoregulation) [Pubmed|22238377]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B836 (yybR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1835 NBRP B. subtilis, Japan]
  • BKE40540 (Δ[gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT
  • BKK40540 (Δ[gene|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT

    • Labs working on this gene/protein

  • [SW|Haike Antelmann],University of Greifswald, Germany